Development and Optimization of a Method for Organochlorine Pesticides Determination in Mussels Based on Miniaturized Matrix Solid-Phase Dispersion Combined with Gas Chromatography–Tandem Mass Spectrometry
| Autor: | N. Carro, A. Mouteira, Ángel López, I. García, M. Ignacio, J. Cobas |
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| Rok vydání: | 2021 |
| Předmět: |
Chromatography
Gas Chromatography/Tandem Mass Spectrometry Chemistry 010401 analytical chemistry Extraction (chemistry) Fractional factorial design 010402 general chemistry Mass spectrometry 01 natural sciences 0104 chemical sciences Analytical Chemistry Matrix (chemical analysis) Solvent Linear range Dispersion (chemistry) |
| Zdroj: | Journal of Analytical Chemistry. 76:603-612 |
| ISSN: | 1608-3199 1061-9348 |
| DOI: | 10.1134/s1061934821050099 |
| Popis: | A miniaturized methodology was developed and applied to the determination of fourteen organochlorine pesticides (OCPs) in mussel samples. This approach was based on matrix solid phase dispersion that enabled to perform extraction and clean-up of the sample at the same step. Subsequently, the extracts were readily analyzed by gas chromatography−tandem mass spectrometry. The extraction and clean-up parameters, amounts of sample, drying agent (anhydrous sodium sulfate) and dispersant agent (Florisil) and solvent volume and polarity (mixture) were optimized by using a fractional factorial design 25–1. Four of five factors, namely, sample and Florisil amounts, solvent volume and solvent mixture, were statistically significant for some of OCPs. The analytical performance demonstrated a broad linear range (R2 < 0.998), recoveries around 85% and relative standard deviation below 7%. The methodology was applied to the analysis of three mussel samples coming from Rias of Ferrol and A Coruna in Galicia coast (NW, Spain). |
| Databáze: | OpenAIRE |
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