| Autor: |
Pei Ying Ng, Amy B.P. Ribet, Qiang Guo, Benjamin H. Mullin, Jamie W.Y. Tan, Euphemie Landao-Bassonga, Sébastien Stephens, Kai Chen, Laila Abudulai, Maike Bollen, Edward T.T.T. Nguyen, Jasreen Kular, John M. Papadimitriou, Kent Søe, Rohan D. Teasdale, Jiake Xu, Robert G. Parton, Hiroshi Takanayagi, Nathan J. Pavlos |
| Rok vydání: |
2022 |
| DOI: |
10.1101/2022.04.28.489831 |
| Popis: |
Osteoclasts are giant bone-digesting cells that harbour specialized lysosome-related organelles termed secretory lysosomes (SLs). SLs store cathepsin K and serve as a membrane precursor to the ruffled border, the osteoclast’s ‘resorptive apparatus’. Yet, the molecular composition and spatiotemporal organization of SLs remains incompletely understood. Here, using organelle-resolution proteomics, we identify member a2 of the solute carrier 37 family (Slc37a2) as a SL sugar transporter. We demonstrate that Slc37a2 localizes to the SL limiting membrane and that these organelles adopt a hitherto unnoticed but dynamic tubular network in living osteoclasts that is required for bone digestion. Accordingly, mice lacking Slc37a2 accrue high bone mass owing to uncoupled bone metabolism and disturbances in SL export of monosaccharide sugars, a prerequisite for SL delivery to the ruffled border. Thus, Slc37a2 is a physiological component of the osteoclast’s unique secretory organelle and a potential therapeutic target for metabolic bone diseases. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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