NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®) E7658Express Protocol without PCR Bead Cleanup v1

Autor:	New England Biolabs
Rok vydání:	2021
Předmět:	Bead (woodworking) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Biology Virology
DOI:	10.17504/protocols.io.bvufn6tn
Popis:	This protocol details methods for the NEBNext® ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina®), NEB #E7658S/L 24/96 reactions. This protocol does not include a cleanup step for each sample after cDNA synthesis and ligation. Performing the cleanup step creates libraries with higher yields and less likelihood for adaptor dimer. Skipping these steps reduces hands on time, but may require an extra cleanup at the end of library prep of the pooled sample and we have added an extra PCR cycle.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c6b3caf2ceb4a29136085d04db35f606 https://doi.org/10.17504/protocols.io.bvufn6tn Zobrazit plný text záznamu