Congruence of SQMl Protein Expression with Methotrexate Sensitivity and Transport
| Autor: | Yuk-Chor Wong, Samuel D. Bernal, R. S. De Villa |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
musculoskeletal diseases
Cancer Research Binding protein General Medicine Membrane transport Biology In vitro Squamous carcinoma chemistry.chemical_compound Oncology Membrane protein chemistry Biochemistry immune system diseases Cell culture Antifolate heterocyclic compounds skin and connective tissue diseases Polyglutamylation |
| Zdroj: | Cancer Investigation. 13:23-30 |
| ISSN: | 1532-4192 0735-7907 |
| DOI: | 10.3109/07357909509024891 |
| Popis: | Expression of SQM1 protein, a membrane protein, was shown to be reduced in human squamous carcinoma of the head and neck (SqCHN) cell lines made less sensitive to methotrexate (MTX). High correlation of SQM1 protein expression with MTX transport (r =.94) and MTX sensitivity (r =.94) was found in MTX-resistant sublines developed from a clonally drived SqCHN cell line, SCC 1551. Unlike SQM1 protein, there was no significant difference in amount or molecular weight of reduced-folate binding protein found in the membrane of a MTX-resistant subline, SCC15R1–3 and SCCI5S1. Furthermore, MTX surface membrane binding was not significantly altered in SCC15Rl-3. Compared to SCC15S1 parent cell line, SCCI5RI-3 subline with similar DHFR enzyme level and MTX polyglutamylation showed a marked reduction in MTX uptake due to a decrease in Vmax without a significant change in Kt. These findings suggest the existence of membrane molecules like SQM1 protein that may indirectly affect MTX transport. |
| Databáze: | OpenAIRE |
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