| Autor: |
Eiman Soliman, Hanan Mahrous, Sally Tayel, Manal Tayel |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Egyptian Journal of Rheumatology and Clinical Immunology. 3:59-66 |
| ISSN: |
2357-0970 |
| Popis: |
Objectives: To assess the chromosomal aberrations (CAs) as a measure of cytotoxicity and micronuclei (MNi) andnucleoplasmic bridges (NPB) as a measure of genotoxicity in patients with rheumatoid arthritis (RA) and its relation to thedisease activity. The work also aimed to assess the possible cytotoxicity and genotoxicity induced by some DiseaseModifying Anti-Rheumatic Drugs (DMARDs) used for treatment of RA patients. Patients and Methods: The study included20 female RA patients treated with DMARDs (MTX and SSZ), 10 RA patients not receiving DMARDs (used NDAIDs), and10 control. Peripheral blood samples were taken to assess the cytotoxicity by chromosomal analysis (karyotyping) using solid Giemsa stain and GTG- banding according to the International System for Human Chromosome Nomenclature. Genotoxicity and DNA damage was assessed by the miconucleus (MN) test using cytochalasin-B. Results: The DMARDs RA patients had a mean age of 42.2±11.76 yr and a mean disease duration of 7.95±6.43 yr. The MTX dose was 50 mg/wk and the SSZ dose was 500 mg/ 8 hours, and the mean duration of use was 7.1±6.27 yr. The 20 RA patients of the DMARDs group received folic acid dose of 0.5 mg/day for the same period as DMARDs. The mean age of the non- MTX RA (NSAIDs) group was 43.1±13.5 yr, the mean disease duration was 4.3±1.9 yr. The results showed a high significant increase (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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