Heterogeneity of Microvascular Endothelial Cells of the Brain: A Comparison of the Effects of Extracellular Matrix and Soluble Astrocytic Factors
| Autor: | Lucienne Juillerat-Jeanneret, Pushpa Darekar And, Robert-Charles Janzer |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
education.field_of_study
Physiology Population Cell Biology General Medicine Biology Blood–brain barrier Cell biology Endothelial stem cell Vascular endothelial growth factor B Extracellular matrix Vascular endothelial growth factor A Vasculogenesis medicine.anatomical_structure Cell culture medicine education |
| Zdroj: | Endothelium. 1:85-91 |
| ISSN: | 1029-2373 1062-3329 |
| DOI: | 10.3109/10623329309102314 |
| Popis: | Until recently, microvascular endothelial cells of the blood-brain barrier were considered a homogeneous population, whose properties were induced by astrocytes. To address the question of brain endothelial cell heterogeneity, we measured the activity of specific markers of endothelial cells of different origins, including two brain-derived endothelial cell lines grown on plastic or on extracellular matrix (ECM) components secreted either by a glial or a fibroblast cell line, with or without the addition of astrocyte-conditioned medium (ACM).ECM components and ACM conjointly, but differently in each endothelial cell line, increased the uptake of leucine and modulated the expression of the specific markers alkaline phosphatase (ALP) and gamma-glutamyl transpeptidase (GGTP) in both cerebral endothelial lines. ACM decreased the incorporation of thymidine in the pulmonary endothelial cell line, but not in a cerebral cell line. An induction of blood-brain barrier specific GGTP was never observed in endothelial... |
| Databáze: | OpenAIRE |
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