Modification of Cas9, gRNA and PAM: Key to further regulate genome editing and its applications
| Autor: | V Ravichandiran, Dhritiman Dey, Syamal Roy, Dipanjan Ghosh, Darshana Gupta, Rahul Gupta, Snehasikta Swarnakar, Rajveer Singh, Kazi Tawsif Ahmed |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Computer science Cas9 Computational biology Genome engineering 03 medical and health sciences Protospacer adjacent motif ComputingMethodologies_PATTERNRECOGNITION 030104 developmental biology 0302 clinical medicine Genome editing 030220 oncology & carcinogenesis Key (cryptography) CRISPR Guide RNA |
| Zdroj: | Progress in Molecular Biology and Translational Science ISBN: 9780128215906 |
| DOI: | 10.1016/bs.pmbts.2020.12.001 |
| Popis: | The discovery of CRISPR-Cas9 system has revolutionized the genome engineering research and has been established as a gold standard genome editing platform. This system has found its application in biochemical researches as well as in medical fields including disease diagnosis, development of therapeutics, etc. The enormous versatility of the CRISPR-Cas9 as a high throughput genome engineering platform, is derailed by its off-target activity. To overcome this, researchers from all over the globe have explored the system structurally and functionally and postulated several strategies to upgrade the system components including redesigning of Cas9 Nuclease and modification of guide RNA(gRNA) structure and customization of the protospacer adjacent motif. Here in this review, we portray the comprehensive overview of the strategies that has been adopted for redesigning the CRISPR-Cas9 system to enhance the efficiency and fidelity of the technology. |
| Databáze: | OpenAIRE |
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