The Ratio between Lethal and Mutagenic Damages in the DNA of Plasmids and Bacteriophages Induced by 8-Methoxypsoralen Plus UV (λ ≥ 320 nm) Treatment

Autor: V. Yu. Kotova, Gennadii B. Zavilgelsky, S. K. Abilev
Rok vydání: 2021
Předmět:
Zdroj: Russian Journal of Genetics. 57:795-803
ISSN: 1608-3369
1022-7954
DOI: 10.1134/s1022795421070097
Popis: The K-ratio between diadducts (D) and monoadducts (M) induced by 8-methoxypsoralen (8-MOP) in the DNA packed in the head of bacteriophage λ and in the DNA of pBR322 plasmid under UV irradiation (λ ≥ 320 nm) was measured. The probabilities of UVR excision repair of 8-MOP monoadducts (P) and of SOS repair of 8-MOP diadducts and monoadducts (S and Sm) were determined. P was measured using an angular derivative of angelicin. It was demonstrated that P = 0.86. S and Smwere determined using bacteriophage λ11 treated with 8-MOP + UV (λ ≥ 320 nm) or 8-MOP + UV (λ ≥ 380 nm) and inoculated either onto bacteria pre-irradiated with short-wave UV light (λ = 254 nm) or onto bacteria with constitutive synthesis of the SOS regulon genes, as well as onto bacteria containing the pKM101 plasmid. In bacteriophage λ11, the level of W-reactivation (α) and the frequency of W-mutagenesis of clear mutations (m) were determined. It was demonstrated that 8-MOP diadducts (“crosslinks”) were repaired by the bacterial SOS system with the probability S = 0.28–0.29, and 8-MOP monoadducts were repaired by the bacterial SOS system with the probability Sm = 0.41 only with the participation of the MucA’2B enzyme, the genes for which were located in the conjugative plasmid pKM101.
Databáze: OpenAIRE
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