Bactericidal action of slightly acidic electrolyzed water against Escherichia coli and Staphylococcus aureus via multiple cell targets
| Autor: | Xingqian Ye, Shiguo Chen, Jiao Li, Xinyu Liao, Tian Ding, Yuanjie Suo, Donghong Liu, Xiao-Ting Xuan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species Hypochlorous acid Microorganism 04 agricultural and veterinary sciences Biology medicine.disease_cause 040401 food science chemistry.chemical_compound 0404 agricultural biotechnology Enzyme Biochemistry chemistry Staphylococcus aureus medicine Bacterial outer membrane Escherichia coli Intracellular Food Science Biotechnology |
| Zdroj: | Food Control. 79:380-385 |
| ISSN: | 0956-7135 |
| Popis: | This study combined plate counting method and fluorescent techniques (membrane integrity and potential, intracellular enzyme activity, and intracellular ROS level) to investigate the lethal and sublethal effects of slightly acidic electrolyzed water (SAEW) on Escherichia coli and Staphylococcus aureus . Also, the inactivation mechanism of SAEW was further explored through multiple cell targets (outer membrane and intracellular components). The results within 30 s SAEW treatment displayed 6.02 and 5.83 log reductions obtained for E. coli and S. aureus , respectively. The maximum sublethally injured cell proportions induced by SAEW exposure were 0.34 and 0.40 log 10 CFU/mL for E. coli and S. aureus , respectively. According to the data from experiments of various acting cellular sites by fluorescent techniques, SAEW damaged the microbial membrane integrity and membrane potential severely. Also, it posed inactivation effect on the activity of intracellular esterase enzymes. Therefore, SAEW showed disinfection behavior with multiple cellular targets, including both cell barriers and intracellular components. Furthermore, SAEW did not result in accumulation of reactive oxygen species (ROS) inside microbial cell, indicating SAEW conducted a ROS-independent behavior on microbial inactivation and the chemical oxidants (e.g., hypochlorous acid) played major role in microbial intracellular oxidation processing. The result in this study will help to further understand the disinfection mechanism underlying SAEW on microorganisms and make SAEW inactivation targets more explicit. |
| Databáze: | OpenAIRE |
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