Preparation and Characterization of Fe3O4@MTX Magnetic Nanoparticles for Thermochemotherapy of Primary Central Nervous System Lymphoma in vitro and in vivo
| Autor: | Yan Zhang, Tian Xue, Zhong Yuejiao, Yao Jingqing, Qianling Lu, Tingting Bai, Li Yuntao, Zhirui Guo, Dai Xinyu |
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| Rok vydání: | 2019 |
| Předmět: |
Hyperthermia
medicine.medical_treatment Biophysics Pharmaceutical Science Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Flow cytometry Biomaterials immune system diseases In vivo hemic and lymphatic diseases Drug Discovery Medicine Chemotherapy medicine.diagnostic_test business.industry Organic Chemistry Primary central nervous system lymphoma General Medicine 021001 nanoscience & nanotechnology medicine.disease 0104 chemical sciences Lymphoma Apoptosis Tumor progression Cancer research 0210 nano-technology business |
| Zdroj: | International Journal of Nanomedicine. 14:9647-9663 |
| ISSN: | 1178-2013 |
| Popis: | Background Primary central nervous system lymphomas (PCNSL) are extranodal malignant non-Hodgkin lymphomas (NHL) that arise exclusively in central nervous system (CNS). Diffuse large B-cell lymphoma (DLBCL) is the most common histological subtype. Purpose To evaluate whether nano drug-loading system-mediated magnetic-targeted thermochemotherapy could produce a better therapeutic effect than single chemotherapy while reducing the use of chemotherapeutic drugs. Methods Six groups (control, Fe3O4, MTX, Fe3O4@MTX, Fe3O4 with hyperthermia and Fe3O4@MTX with hyperthermia) were set. Tumor cell apoptosis in each treatment group was detected by flow cytometry. Apoptosis-related gene expressions Caspase-3, Bax and Bcl-2 were detected by qPCR and Western blot; intracranial tumor model of PCNSL was established by intracranial injection of OCI-LY18 tumor cells into BALB/c-Nude mice. Magnetic resonance imaging (MRI) was used to monitor tumor progression and HE tumor cells were significantly reduced in thermochemotherapy group. Expression of apoptosis-related gene Caspase-3 and Bax were significantly upregulated in tumor tissues, while expression of Bcl-2 gene was significantly downregulated. Conclusion These results demonstrated in vivo and in vitro that the combined thermochemotherapy of Fe3O4@MTX MNPs was superior to the single MTX chemotherapy with less dosage, which may promote apoptosis of DLBCL cells through the mitochondrial apoptotic pathway and provided a new way for the treatment of PCNSL. |
| Databáze: | OpenAIRE |
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