Separation and direct detection of raw and gelatinized starch hydrolyzing activities of glucoamylase on isoelectric focusing gels
| Autor: | Sukumaran Umesh-Kumar, Naikantatte G. Karanth, Ashok Kumar Dubey, Cuddapah Suresh, Ramachandra K. Kini |
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| Rok vydání: | 1999 |
| Předmět: |
Chromatography
food.ingredient biology Starch Isoelectric focusing Clinical Biochemistry Aspergillus niger food and beverages Substrate (chemistry) Protein engineering biology.organism_classification Biochemistry Gelatin Analytical Chemistry chemistry.chemical_compound Hydrolysis food chemistry Reagent |
| Zdroj: | Electrophoresis. 20:483-485 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/(sici)1522-2683(19990301)20:3<483::aid-elps483>3.0.co;2-b |
| Popis: | A procedure to detect raw and gelatinized starch activities of glucoamylase on isoelectric focusing (IEF) gels by using 2, 3, 5-triphenyltetrazolium chloride is described. The reagent reacts with the reducing group of glucose released by glucoamylase from the substrate starch. Using the reaction, production of glucoamylase by Aspergillus niger was detected on 10% IEF gels within a pH range of 2.5-9.5. Since the method can detect raw and gelatinized starch activities of glucomylase associated with 1 microg protein, it will be useful for enzyme engineering studies that involve screening of various mutations. |
| Databáze: | OpenAIRE |
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