Multifunctional PLGA-based nanoparticles as a controlled release drug delivery system for antioxidant and anticoagulant therapy
| Autor: | Pei-Chi Lee, Tze-Wen Chung, Li-Ting Chen, Bo-Shen Zan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Biophysics
Pharmaceutical Science Fibroin Bioengineering 02 engineering and technology Pharmacology 010402 general chemistry 01 natural sciences Biomaterials Cell therapy chemistry.chemical_compound Drug Discovery Hyaluronic acid medicine Organic Chemistry Mesenchymal stem cell General Medicine Glutathione Heparin 021001 nanoscience & nanotechnology 0104 chemical sciences PLGA chemistry Drug delivery 0210 nano-technology medicine.drug |
| Zdroj: | International Journal of Nanomedicine. 14:1533-1549 |
| ISSN: | 1178-2013 |
| Popis: | Background Ischemia/reperfusion (I/R) injury causes the generation of many ROS such as H2O2 and leads to vascular thrombosis, which causes tissue damage. Purpose In this investigation, poly (lactideco-glycolide) (PLGA)-based nanoparticles are used for their anticoagulant and antioxidant properties in vascular therapy. Methods Both heparin and glutathione are entrapped on PLGA-stearylamine nanoparticles by layer-by-layer interactions. Results The drug release rate is successfully controlled with only 10.3% of the heparin released after 96 hours. An H2O2-responsive platform is also developed by combining silk fibroin and horse peroxidase to detect H2O2 in this drug delivery system. Besides, hyaluronic acid was decorated on the surface of nanoparticles to target the human bone marrow mesenchymal stem cells (hBMSCs) for cell therapy. The results of an in vitro study indicate that the nanoparticles could be taken up by hBMSCs within 2 hours and exocytosis occurred 6 hours after cellular uptake. Conclusion We propose that the multifunctional nanoparticles that are formed herein can be effectively delivered to the site of an I/R injury via the hBMSC homing effect. The proposed approach can potentially be used to treat vascular diseases, providing a platform for hBMSCs for the controlled delivery of a wide range of drugs. |
| Databáze: | OpenAIRE |
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