Effects of low temperature, cold shock, and various carbon sources on esterase and lipase activities and exopolysaccharide production by a psychrotrophicAcinetobactersp
| Autor: | S E Barbaro, J T Trevors, W E Inniss |
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| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | Canadian Journal of Microbiology. 47:194-205 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/w00-141 |
| Popis: | The activities of isocitrate lyase, esterase, and lipase by the psychrotrophic Acinetobacter sp. strain HH1-1 were monitored during incubation at 25°C, 5°C, and after a 25°C to 5°C down shift in growth temperature. During growth at 25°C, isocitrate lyase activity was detected in cell-free extracts, but at 5°C and after cold shock, activity was measured primarily in the cell culture supernatant. Strain HH1-1 produced two cell-associated esterases and an extracellular esterase and lipase. Activities of the extracellular esterase and lipase were reduced when cells were grown at 5°C and after cold shock. In contrast, an increased synthesis of a 53-kDa cell-associated esterase was observed 50 h after cold shock. An extracellular polysaccharide was also produced, indicated by a decrease in surface tension in cell culture supernatant when cells were incubated at 25°C; but like extracellular enzyme activity, production of the exopolymer was reduced when cells were subjected to low temperatures. These results indicated that the intracellular enzyme, isocitrate lyase, leaked out of the cell after cold shock and during growth at 5°C. The increased activity of a cell-associated esterase suggested this enzyme is required for growth at low temperatures. In contrast, activities of extracellular lipolytic enzymes and production of an extracellular polysaccharide were negatively affected at the lower temperatures.Key words: Acinetobacter, cold shock, esterase, lipase, exopolysaccharide. |
| Databáze: | OpenAIRE |
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