Use of Spider Venom and Botanicals as Acetylcholinesterase Inhibitors Against Musca domestica (L.) (Diptera: Muscidae) and Aedes (Stegomyia) Aegypti L. (Dipetra: Culicidae)
| Autor: | Junaid Nadeem, Ali Hassan, Hafiz Muhammad Tahir, Azhar Abbas Khan, Zeshan Hassan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Aedes biology Traditional medicine Mentha arvensis 010607 zoology Venom Aedes aegypti biology.organism_classification 01 natural sciences 010602 entomology Insect Science Muscidae Eucalyptus globulus Bioassay Agronomy and Crop Science Musca Ecology Evolution Behavior and Systematics |
| Zdroj: | African Entomology. 27:366 |
| ISSN: | 1021-3589 |
| DOI: | 10.4001/003.027.0366 |
| Popis: | The aim of the study was to determine insecticidal potential of three plant extracts (Calatropis procera, Eucalyptus globulus and Mentha arvensis) and crude venom of an orb-web spider, Neoscona theisi, in the laboratory using Musca domestica and Aedes aegypti as model insects. Laboratory bioassays were conducted against three doses of plants extracts (0.2 mg/ml, 0.4 mg/ml and 0.6 mg/ml) and spider venom (10 µg/ml, 20 µg/ml and 30 µg/ml). Ellman et al.'s (1961) method was followed to estimate the activity of acetylcholinesterase of insects. Calatropis procera was found to be more effective in killing the insects. The mortality rate was increased with an increase in plant extract or venom dose. Calatropis procera inhibited acetylcholinesterase more proficiently as compared to the M. arvensis and E. globulus extracts. Crude spider venom also significantly inhibited the estimated activity of acetylcholinesterase in M. domestica and Ae. aegypti. It is concluded from the study that both plant extracts and spider venom inhibited the activity of acetylcholinesterase in M. domestica and Ae. aegypti but C. procera is more efficient in killing the insects. |
| Databáze: | OpenAIRE |
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