Enzyme-Linked Immunoassay for Detection of Listeria monocytogenes in Dairy Products, Seafoods, and Meats: Collaborative Study
| Autor: | Wendy A. Lepper, Michael S Curiale, Barbara J Robison |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Pharmacology
biology medicine.diagnostic_test business.industry food and beverages medicine.disease_cause biology.organism_classification Food safety food.food Analytical Chemistry Food and drug administration food Listeria monocytogenes Immunoassay medicine Listeria Roast beef Environmental Chemistry Enzyme linked immunoassay Food science Elisa method business Agronomy and Crop Science Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 77:1472-1489 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoac/77.6.1472 |
| Popis: | A collaborative study was conducted to evaluate Listeria-TekTM, an enzyme-linked immunosorbent assay (ELISA) for detection of Listeria monocytogenes and other Listeria spp. in foods. The present ELISA method was compared to the U.S. Food and Drug Administration culture method for detection of L. monocytogenes in dairy products and seafoods and to the U.S. Department of Agriculture Food Safety and Inspection Service method for detection of L. monocytogenes in meats. Replicate samples of 6 food types (frankfurters, roast beef, Brie cheese, 2% milk, raw shrimp, and crab meat) inoculated with L. monocytogenes and uninoculated control samples were analyzed by the collaborators. L. monocytogenes was identified in 593 samples by the ELISA method and in 574 samples using culture procedures. Identical results were obtained for 506 positive samples and 419 negative samples using the ELISA and culture methods for an overall agreement rate of 85.6%. The enzyme-linked immunoassay for detection of L. monocytogenes in dairy, seafood, and meat products has been adopted first action by AOAC INTERNATIONAL. |
| Databáze: | OpenAIRE |
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