Evaluation of Fumonitest Immunoaffinity Columns
| Autor: | George M Ware, Allen S Carman, Pesi P. Umrigar, Shia S Kuan |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Chromatography
Elution Biochemistry (medical) Clinical Biochemistry Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound chemistry Affinity chromatography Fumonisin Electrochemistry Methanol Isoindole Derivatization Spectroscopy |
| Zdroj: | Analytical Letters. 27:693-715 |
| ISSN: | 1532-236X 0003-2719 |
| Popis: | A method for the determination of fumonisins B1 and B2 in corn was developed. The method involves sample extraction with methanol:water (80:20) and the use of a commercially available Fumonitest column for sample cleanup. The capacity, selectivity, column-to-column and lot-to-lot reproducibility of the Fumonitest columns were evaluated. The total capacity of the column was found to be 1.2 μg fumonisin. Both fumonisins B1 and B2 had an equal affinity toward the Fumonitest column, with the sample matrix demonstrating little effect on the column performance. The maximum sample size was 0.5 g for samples containing total fumonisins of less than 2 ppm. After elution from the immunoaffinity column, fumonisins B1 and B2 were reacted with naphthalene-2, 3-dicarboxaldehyde (NDA) to produce a highly fluorescent derivative, 1-cyano-2-alkyl-benz[f]isoindole (CBI). The derivatives were then separated from the sample matrix on a reverse phase C-18 column with a mobile phase consisting of acetonitrile:water:ace... |
| Databáze: | OpenAIRE |
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