Reducing the microcapsule diameter by micro-emulsion to improve the insecticidal activity ofBacillus thuringiensisencapsulated formulations

Autor: Lucila Valdez-Castro, Laura Lina-Garcia, Dulce S. Salgado-Urias, Omar Solorza-Feria, Josefina Barrera-Cortés
Rok vydání: 2016
Předmět:
Zdroj: Biocontrol Science and Technology. 27:42-57
ISSN: 1360-0478
0958-3157
DOI: 10.1080/09583157.2016.1244258
Popis: The emulsion/internal gelation method is highly effective to produce microcapsules of Bacillus thuringiensis (Bt) in a short time; however, it has the limitation to produce microcapsules within a wide range of diameters (1–1000 µm). The aim of this study was to reduce the range of small microcapsule diameters by using a water/corn-oil (W/CO) micro-emulsion as the dispersing medium and the mixture Tween 80–Span 80 as the surfactant. It involved the development of the W/CO micro-emulsion and the determination of the suitable agitation time to disperse the gelling medium (sodium alginate) through the micro-emulsion. A micro-emulsion formulation that allowed reduction of the microcapsule diameter was composed of 82% corn oil, 12% alginate solution and 6% surfactant mixture Tween80–Span80 (31:69). Evaluation of four dispersing times showed that 45 min was suitable to produce 75% of microcapsules of an average diameter of 3.1 ± 1 µm containing the spore–protein complex (SPC) produced by Bt. Bioassays ca...
Databáze: OpenAIRE