A Putative RAPD Marker for Common Bunt Resistance in Wheat Dihaploid Lines
| Autor: | Constantin Botez, M. A. Grigoras, Laura Cristina Cota, Dana Curticiu |
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| Rok vydání: | 1970 |
| Předmět: | |
| Zdroj: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Agriculture. 66 |
| ISSN: | 1843-5386 1843-5246 |
| DOI: | 10.15835/buasvmcn-agr:3940 |
| Popis: | The present paper presents the results of the genetic analyses by testing some RAPD primers, trying to find molecular markers linked to wheat common bunt resistance caused by Tilletia spp. Dihaploid lines (DH) derived from two crosses were tested and their genealogy is: GP369=99419G4-1A/1-1/00356G8-1 and GP 384 GP384=99419G4-1A/1-1/98047G1421INC1 (obtained from NARDI Fundulea). These lines have in their genealogy sources of resistance against Tilletia species which come from parental form (line 99419G4) known as very resistant to Tilletia. The testing was carried out comparatively to eight susceptible cultivars, out of which Dropia cultivar is known as very susceptible to Tilletia (Oncica and Saulescu, 2007). The RAPD polymorphic fragment of 590 bp was identified with Mic 13 primer (5'-TTCCCCCCAG-3') and it probably marks a certain resistance gene to Tilletia because this was absent in all eight susceptible tested cultivars (Fig. 1). The fragment of 590 bp was present in several DH lines from GP 348 cross, but also in some DH lines from 369 cross. (Fig. 1 and Fig. 2). But the polymorphic fragment was absent at the parental form (line 99419G4), for which some polymorphic fragments with some RAPD primers were identified previously. It is possible that the resistance of this dihaploid lines (DH) to Tilletia spp. come from other lines like 00356G8-1 or 98047G14, about which we do not have yet data referring to the resistance gene or to the phenotypic characterization of resistance to Tilletia spp. |
| Databáze: | OpenAIRE |
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