| Autor: |
Nicolas Noiseux, Melanie Borie, L.M. Stevens, S. DerSarkissian, Denis-Claude Roy, Samer Mansour, M. Vu |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Canadian Journal of Cardiology. 30:S55 |
| ISSN: |
0828-282X |
| DOI: |
10.1016/j.cjca.2014.07.020 |
| Popis: |
BACKGROUND: An important issue limiting cell therapy in the treatment of infarcted heart is the extensive loss of transplanted cells. Manipulation of the cells prior to the transplantation has been proposed in order to improve their biologic and functional properties through enhancement of cell survival, homing, retention, differentiation or proliferation. METHODS: The objective was to optimize human stem cells using a novel strategy of preconditioning with oxytocin (OT) and their preparation for clinical applications with improved viability and therapeutic function. Human mesenchymal stem cells (hMSC) were treated with OT from 0.1 nM to 1 mM (range of physiological and pharmacological doses) with either short bursts or continuously to determine the conditions with highest responsiveness. OT signalling pathways were investigated according to phosphorylation of proteins or activation of specific effectors. The LIVE/DEAD assay kit assessed cellular viability. Multilineage differentiation was confirmed by in vitro assays for tri-potentiality based on adipogenesis (oil red staining) and osteogenesis (alizarin staining). Phenotype and surface markers were evaluated by standard fluorescence-activated cell sorting techniques to validate changes in protein expression following OT treatment at different time points/conditions. RESULTS: OT receptor mRNAwas found in hMSC by RT-PCR and proteins byWestern blotting.OT rapidly (5-15minutes) and significantly increased phosphorylation of PI3K/AKT (1,6 fold) and ERK1/2 (3,0 folds) all P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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