| Popis: |
A liquid chromatographic method that can separate each of a series of diamino analogues of 2′- or 3′-deoxyadenosine from their main degradation product, adenine, is described. A PLRP-S 1000 A (8 μm) 250 mm × 4.6 mm. I.D. column was used at 60°C. The method was developed by systematic evaluation of the influence of the mobile phase pH, the type and concentration of the organic modifier, the concentration of the ion-pairing agent and the buffer. The mobile phase consisted of tetrahydrofuran-0.2 M sodium octanesulphonate-0.2 M potassium phosphate buffer (pH 2.0)-water (15:25:30:30, v/v). |
