Microchip device for measurement of body volume of C. elegans as bioindicator application

Autor: Masaru Kojima, Katsutoshi Ooe, Masahiro Nakajima, Jaehoon Jung, Toshio Fukuda
Rok vydání: 2011
Předmět:
Zdroj: Journal of Micro-Nano Mechatronics. 7:3-11
ISSN: 1865-3936
1865-3928
DOI: 10.1007/s12213-011-0036-7
Popis: We propose a micro fluidic device to analyze Caenorhabditis elegans (C. elegans) as a bioindicator. In this device, we assessed the body volume of C. elegans using a microchip with a capacitance sensor. This device is composed of two key parts. One is a tapered micro channel. The tapered micro channel is used to immobilize C. elegans. The other is a pair of electrodes. The electrodes are used to measure the capacitance change. These two components form a sensing space; a capacitance change occurs when C. elegans is placed in the sensing space. In this study, we performed two kinds of experiments. First, to check the correlation between the body volume and the capacitance change, a total of 15 C. elegans were measured; from L2 larval stage to adult stage. Second, to confirm the possibility of the proposed microchip as a device that can analyze the condition of C. elegans as a bioindicator, a total of 35 C. elegans were measured. To measure the body volume as a reference, first, the body length and the diameter of C. elegans were measured by an optical microscope and then the volume was calculated from those two measured values. The capacitance change was measured by the proposed microchip. By comparison between the result of two methods, we confirmed the correlation between the calculated values. The possibility of the proposed microchip as the device to analyze the effect of cadmium on C. elegans as a bioindicator was established.
Databáze: OpenAIRE
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