Transfection of the Giardia lamblia double-stranded RNA virus into giardia lamblia by electroporation of a single-stranded RNA copy of the viral genome
Autor: | E S Furfine, Ching C. Wang |
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Rok vydání: | 1990 |
Předmět: |
animal structures
biology viruses RNA RNA virus Cell Biology Transfection biology.organism_classification medicine.disease_cause Virology Molecular biology Virus embryonic structures parasitic diseases Nucleic acid medicine Double-stranded RNA viruses Giardia lamblia Molecular Biology Single-Stranded RNA |
Zdroj: | Molecular and Cellular Biology. 10:3659-3662 |
ISSN: | 1098-5549 0270-7306 |
Popis: | The development of a genetic vector for protozoan parasites is a major hurdle yet to be crossed in the study of the molecular and cellular biology of these parasites. We have identified and isolated a double-stranded RNA virus (G. lamblia virus [GLV]) from certain strains of the intestinal parasitic protozoan Giardia lamblia (A. L. Wang and C. C. Wang, Mol. Biochem. Parasitol. 21:269-276, 1986), which is capable of infecting other virus-free strains of G. lamblia (R. L. Miller, A. L. Wang, and C. C. Wang, Exp. Parasitol. 66:118-123, 1988). Here we demonstrate that G. lamblia can be infected with GLV by electroporating uninfected cells with purified single-stranded RNA (E. S. Furfine, T. C. White, A. L. Wang, and C. C. Wang, Nucleic Acids Res. 17:7453-7467, 1989) representing a full-length copy of one strand of the GLV double-stranded RNA genome. To the best of our knowledge, this is the first demonstration in vivo that a single-stranded RNA is a competent replicative intermediate for this class of double-stranded RNA virus. In addition, this result represents the first long-term transfection of a protozoan by a single species of RNA and will hopefully expedite the development of GLV as a genetic transfecting vector. |
Databáze: | OpenAIRE |
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