Metabolism of [14C]Prometryn in Rats
| Autor: | Randy L. Rose, William Itterly, Diane Brumback, Michael S. Maynard, Thomas M. Capps |
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| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | Journal of Agricultural and Food Chemistry. 47:3858-3865 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf981363+ |
| Popis: | [(1)(4)C]Prometryn, 2, 4-bis(isopropylamino)-6-(methylthio)-s-triazine, was orally administered to male and female rats at approximately 0.5 and 500 mg/kg; daily urine and feces were collected. After 3 or 7 days rats were sacrificed, and blood and selected tissues were isolated. The urine and feces extracts were characterized for metabolite similarity as well as for metabolite identification. Over 30 metabolites were observed, and of these, 28 were identified mostly by mass spectrometry and/or cochromatography with available reference standards. The metabolism of prometryn was shown to occur by N-demethylation, S-oxidation, S-S dimerization, OH substitution for NH(2) and SCH(3), and conjugation with glutathione or glucuronic acid. Rat liver microsomal incubations of prometryn were conducted and compared to the in vivo metabolism. Both in vivo and in vitro phase I metabolisms of prometryn were similar, with S-oxidation and N-dealkylation predominating. The involvement of cytochrome P-450 and flavin-containing monooxidase in the in vitro metabolism of prometryn was investigated. |
| Databáze: | OpenAIRE |
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