Timing ofin vivomaturation of equine preovulatory oocytes and competence forin vitromaturation of immature oocytes collected simultaneously

Autor:	Anna Mekarska, Eric Palmer, J. Bezard, Ghylène Goudet, Guy Duchamp
Rok vydání:	1997
Předmět:	medicine.medical_specialty media_common.quotation_subject General Medicine Biology Oocyte In vitro In vitro maturation Endocrinology medicine.anatomical_structure In vivo Internal medicine Follicular phase medicine Metaphase i Ovulation media_common
Zdroj:	Equine Veterinary Journal. 29:33-37
ISSN:	0425-1644
DOI:	10.1111/j.2042-3306.1997.tb05096.x
Popis:	The objects of this study were to monitor the development of the cumulus complex and nuclear maturation in oocytes recovered from preovulatory follicles following treatment to induce ovulation and to investigate the in vitro maturation competence of oocytes recovered from smaller nonpreovulatory follicles of varying size. All follicles > or =5 mm in pony mares were individually punctured at 0, 6, 12, 24 and 35 h after an injection of LH to induce ovulation. The recovery rates of oocytes were 64% from 55 preovulatory follicles, 22% from 32 subordinate follicles and 52% from 227 small follicles. Cumulus expansion of the preovulatory oocytes occurred at 12 h post LH treatment while the metaphase I and II components of nuclear maturation were not completed until 24 and 35 h post LH respectively. For nonpreovulatory follicles, the frequency of atresia and oocyte competence for in vitro nuclear maturation both increased with increasing follicular size.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c58f18d53123d7e31499f38ce4d3aee5 https://doi.org/10.1111/j.2042-3306.1997.tb05096.x Zobrazit plný text záznamu Plný text