Cellulose hydrogel film for spheroid formation of human adipose-derived stemcells
| Autor: | Hyeon Joo Kim, Tong Ho Kang, Masahisa Wada, Rodrigo Castañeda, Satoshi Kimura, Ung-Jin Kim |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Aqueous solution Polymers and Plastics Chemistry Cellular differentiation Cell Spheroid 02 engineering and technology 021001 nanoscience & nanotechnology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology medicine.anatomical_structure Chemical engineering Tissue engineering embryonic structures medicine Viability assay Cellulose 0210 nano-technology Cytotoxicity |
| Zdroj: | Cellulose. 25:2589-2598 |
| ISSN: | 1572-882X 0969-0239 |
| Popis: | Herein, we present a new process for the formation of cell spheroids in three-dimensional culture systems using cellulose hydrogel film. Transparent cellulose hydrogel film were prepared via dissolution-regeneration with a LiOH/urea aqueous solution and methanol. The cell viability of the cellulose hydrogel film was equivalent to that of cell tissue culture plate, revealing no cytotoxicity. Spheroids of human adipose-derived stem cells (hASCs) were successfully formed on cellulose hydrogel film. Spheroid size strongly depended on the cell seeding density and culture time, becoming larger with increases in both factors. Cell differentiation simultaneously progressed favorably with the spheroid formation of hASCs. These results suggested that cellulose hydrogel film could provide a new option with regard to tissue engineering applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink