Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas
| Autor: | Jean-Baptiste Pettit, Hernando Martínez Vergara, Kaia Achim |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell Image registration High resolution Computational biology In situ hybridization Biology Transcriptome 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Atlas (anatomy) Gene expression medicine Gene 030217 neurology & neurosurgery |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493972128 |
| DOI: | 10.1007/978-1-4939-7213-5_7 |
| Popis: | The method described here aims at the construction of a single-cell resolution gene expression atlas for an animal or tissue, combining in situ hybridization (ISH) and single-cell mRNA-sequencing (scRNAseq).A high resolution and medium-coverage gene expression atlas of an animal or tissue of interest can be obtained by performing a series of ISH experiments, followed by a process of image registration and gene expression averaging. Using the overlapping fraction of the genes, concomitantly obtained scRNAseq data can be fitted into the spatial context of the gene expression atlas, complementing the coverage by genes. |
| Databáze: | OpenAIRE |
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