Angiogenesis is repressed by ethanol exposure during chick embryonic development
| Autor: | Xin Cheng, Wen-hui Lu, Shi-Yao Zhang, Guang Wang, Zheng-lai Ma, Shan Zhong, Jian-long Chen, Da-xiang Lu, Xuesong Yang, Manli Chuai, Kenneth Ka Ho Lee |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Reactive oxygen species Ethanol biology Angiogenesis Embryogenesis Embryo Toxicology Fibroblast growth factor Andrology Vascular endothelial growth factor Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Biochemistry chemistry embryonic structures biology.protein |
| Zdroj: | Journal of Applied Toxicology. 36:692-701 |
| ISSN: | 0260-437X |
| Popis: | It is now known that excess alcohol consumption during pregnancy can cause fetal alcohol syndrome to develop. However, it is not known whether excess ethanol exposure could directly affect angiogenesis in the embryo or angiogenesis being indirectly affected because of ethanol-induced fetal alcohol syndrome. Using the chick yolk sac membrane (YSM) model, we demonstrated that ethanol exposure dramatically inhibited angiogenesis in the YSM of 9-day-old chick embryos, in a dose-dependent manner. Likewise, the anti-angiogenesis effect of ethanol could be seen in the developing vessel plexus (at the same extra-embryonic regions) during earlier stages of embryo development. The anti-angiogenic effect of ethanol was found associated with excess reactive oxygen species (ROS) production; as glutathione peroxidase activity increased while superoxide dismutase 1 and 2 activities decreased in the YSMs. We further validated this observation by exposing chick embryos to 2,2′-azobis-amidinopropane dihydrochloride (a ROS inducer) and obtained a similar anti-angiogenesis effect as ethanol treatment. Semiquantitative reverse transcription–polymerase chain reaction analysis of the experimental YSMs revealed that expression of angiogenesis-related genes, vascular endothelial growth factor and its receptor, fibroblast growth factor 2 and hypoxia-inducible factor, were all repressed following ethanol and 2,2′-azobis-amidinopropane dihydrochloride treatment. In summary, our results suggest that excess ethanol exposure inhibits embryonic angiogenesis through promoting superfluous ROS production during embryo development. Copyright © 2015 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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