Evaluation of the serum level of soluble E -cadherin and cell surface expression of α5β1 integrin in patients with acute leukemia whether lymphocytic or myeloid ,in order to extrapolate their prognostic value (127.3)
| Autor: | Zakia Abdelrahman, Lobna Abo shamaa, Nadia Sadek, Amina El sayed, Nada Mekway |
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| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 188:127.3-127.3 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.188.supp.127.3 |
| Popis: | Background:E-cadherin is expressed on CD34+ stem cell,where it likely contributes to intracellular interactions during hematopoiesis. Also integrin such as α5β1 integrin represent an essential component of the bone marrow microenvironment that regulates cell survival .In leukemia, immature blasts are not retained within the marrow, suggesting a breakdown of adhesive mechanisms and cell migration. Objective: this study was done to evaluate serum soluble E -cadherin and cell surface expression of α5β1 integrin on PBMCs in patients with acute leukemia whether lymphocytic or myeloid Twenty patients (11 AML and 9 ALL)and ten age and sex matched normal individuals were studied. soluble E -cadherin were assayed using ELISA and cell surface expression of α5β1 integrin on PBMCs by indirect immunoflourescence . Results: Soluble E-cadherin was significantly lower in all leukemic and AML patients than control ( p=.0108,p=.0049 respectively).However no significant difference between AML and ALL. α5β1 integrin cell surface expression was significantly higher in both AML,ALL groups than control(p=.0001,0.0038 respectively). E-cadherin has negative significant correlation (r= -.573, p= .008) with bone marrow blast percentage after the induction of chemotherapy. Conclusion this suggests that a dual double way interaction between increased expression of α5β1 integrin and loss or decreased expression of E-cadherin that governs the process of tumour progression and metastasis. |
| Databáze: | OpenAIRE |
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