Co-delivery of curcumin-loaded nanoemulsion and Phaleria macrocarpa extract to NIH 3T3 cell for antifibrosis
| Autor: | Heni Rachmawati, Annisa Rahma, Risya M. Nisa, Catur Riani, Raymond R. Tjandrawinata, Olivia Mayasari Tandrasasmita, Miranti Anggraeni Novel, Guntur Berlian |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science biology Pharmaceutical Science Pharmacology biology.organism_classification 3T3 cells Staining 03 medical and health sciences chemistry.chemical_compound Procollagen peptidase 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Phaleria macrocarpa Biochemistry chemistry 030220 oncology & carcinogenesis medicine Curcumin Fibroblast Sirius Red Drug metabolism |
| Zdroj: | Journal of Drug Delivery Science and Technology. 39:123-130 |
| ISSN: | 1773-2247 |
| DOI: | 10.1016/j.jddst.2017.03.015 |
| Popis: | Antifibrotic effect of curcumin-loaded nanoemulsion and its combination with Phaleria macrocarpa extract (PM) was determined through evaluation on collagen expression in fibroblast NIH/3T3 cells. Prior to activity study, cytotoxic effect of blank nanoemulsion in vitro was determined on different cell types using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. The genes expression of collagens were determined with quantitative real time PCR. The amount of collagen protein was determined using Sirius Red/Fast Green Collagen Staining Kit. Stability of curcumin-loaded nanoemulsion towards hepatic metabolism was evaluated as well. Cells viability was preserved after treatment with curcumin- and curcumin-PM nanoemulsion. Both preparations decreased procollagen α1(1) and collagen IV genes and collagen protein. Nanoemulsion demonstrated protective effect against hepatic metabolism of curcumin. Curcumin nanoemulsion and curcumin-PM nanoemulsion showed a promising antifibrotic agent. |
| Databáze: | OpenAIRE |
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