Identification and evaluation of fragmentation pathways of PDE-5 inhibitor analogues using LC-QTOF-MS
| Autor: | Hyoung-Joon Park, Sung-Kwan Park, Eunyoung Noh, Chang-Yong Yoon, Sooyeul Cho, Soon-Byung Yoon, Jung-Ah Do |
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| Rok vydání: | 2015 |
| Předmět: |
Pharmacology
Chemistry Sildenafil Stereochemistry Electrospray ionization Mass spectrometry Tadalafil Analytical Chemistry Phosphodiesterase Type 5 Inhibitors Lc qtof ms chemistry.chemical_compound Vardenafil Materials Chemistry medicine Environmental Chemistry Fragmentation (cell biology) Agronomy and Crop Science Food Science medicine.drug |
| Zdroj: | Analytical Science and Technology. 28:278-287 |
| ISSN: | 1225-0163 |
| Popis: | Phosphodiesterase type 5 inhibitors (PDE-5 inhibitors) are used in the treatment of erectile dysfunction. In recent years, a number of reports have been conducted on dietary supplements contaminated with PDE-5 analogues. In this study, 58 analogues of PDE-5 inhibitors were sorted into five groups: tadalafil, sildenafil, hongdenafil, vardenafil, and other analogues. These analogues were then evaluated using a liquid chromatographyquadrupole-time of flight mass spectrometry (LC-QTOF-MS) electrospray ionization mass method. Each compound has a unique fragmentation ion, which can be easily analyzed qualitatively. The fragmentation pathways of the analogues were elucidated based on the QTOF-MS and MS/MS data. Common ions were confirmed for each group by analyzing the structural characteristics and fragmentation pathways. Specifically, common ions were observed at m/z 169.08 and 135.04 (tadalafil analogues), m/z 311.15 and 283.12 (sildenafil analogues and hongdenafil analogues), and m/z 312.16 and 151.09 (vardenafil analogues). The advantage of this method is that the structure of unknown components can be determined by interpreting the product ions. Hence, the developed method can be used for the identification of unknown compounds. Fragmentation pathways may also aid in the detection and identification of PDE-5 inhibitor analogues. |
| Databáze: | OpenAIRE |
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