Synthesis ofl -Tryptophan from Indole anddl -Serine by Tryptophan Synthetase Entrapped in Fibres
| Autor: | Francesco Cecere, Walter Marconi, Vincenza Vitobello, Pasquale Zaffaroni, Franco Morisi, Ezio Giacomozzi, Bartoli Francesco |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Indole test
chemistry.chemical_classification Chromatography Tryptophan medicine.disease_cause General Biochemistry Genetics and Molecular Biology Serine chemistry.chemical_compound Cellulose triacetate Enzyme chemistry medicine Glycerol General Agricultural and Biological Sciences Escherichia coli Ammonium sulfate precipitation |
| Zdroj: | Agricultural and Biological Chemistry. 38:1335-1349 |
| ISSN: | 0002-1369 |
| DOI: | 10.1080/00021369.1974.10861333 |
| Popis: | Optimal culture conditions for microbial production of tryptophan synthetase were studied. It was found that on cultivation of Escherichia coli 476, a tryptophan auxotroph, in a medium containing 5g/liter glycerol as C source, supplemented with 1 g/liter of acid-treated peptone, cells with high tryptophan synthetase activity could be obtained.The enzyme was extracted from cells and 3-fold purified by heat treatment and ammonium sulfate precipitation. The overall yield of the isolation procedure was 60%.The partially purified tryptophan synthetase was entrapped in cellulose triacetate fibres. Under storage conditions, in refrigerator, the entrapped enzyme was stable at least for 6 months. The activity of the entrapped enzyme was about 75% with respect to the free enzyme.Similar behaviour for the free and entrapped enzyme was observed as to the effect of temperature and pH on the enzymic activity. The operational stability of the entrapped tryptophan synthetase was very good (activity unchanged after 50 day... |
| Databáze: | OpenAIRE |
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