Enzymatic network for production of ether amines from alcohols
| Autor: | Bian Wu, Rohana Abu, Cyntia M. Palacio, Sebastian Bartsch, Ciprian G. Crismaru, Vaidotas Navickas, Kai Baldenius, Dick B. Janssen, John M. Woodley, Klaus Ditrich, Michael Breuer |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
010405 organic chemistry Transamination Substrate (chemistry) Bioengineering Alcohol Ether 010402 general chemistry 01 natural sciences Applied Microbiology and Biotechnology 0104 chemical sciences Enzyme catalysis Ammonia chemistry.chemical_compound chemistry Biocatalysis Organic chemistry Amination Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 113:1853-1861 |
| ISSN: | 0006-3592 |
| DOI: | 10.1002/bit.25954 |
| Popis: | We constructed an enzymatic network composed of three different enzymes for the synthesis of valuable ether amines. The enzymatic reactions are interconnected to catalyze the oxidation and subsequent transamination of the substrate and to provide cofactor recycling. This allows production of the desired ether amines from the corresponding ether alcohols with inorganic ammonium as the only additional substrate. To examine conversion, individual and overall reaction equilibria were established. Using these data, it was found that the experimentally observed conversions of up to 60% observed for reactions containing 10 mM alcohol and up to 280 mM ammonia corresponded well to predicted conversions. The results indicate that efficient amination can be driven by high concentrations of ammonia and may require improving enzyme robustness for scale-up. Biotechnol. Bioeng. 2016;113: 1853-1861. © 2016 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text