Encapsulation of ribonucleic acid in human red blood cells for use as a reticulocyte quality control material for flow cytometric analysis

Autor: Wayne L. Ryan, Alireza Ebrahim
Rok vydání: 1996
Předmět:
Zdroj: Cytometry. 25:156-163
ISSN: 1097-0320
0196-4763
DOI: 10.1002/(sici)1097-0320(19961001)25:2<156::aid-cyto4>3.0.co;2-f
Popis: The osmotic lysis procedure was employed to en-capsulate ribonucleic acid (RNA) in human red blood cells in order to prepare a reticulocyte reference control. The procedure required the hypotonic dialysis of erythrocytes in the presence of RNA and cytosolic components of red blood cells followed by a short hypertonic dialysis to restore isotonicity and reseal the pores formed on the cell membrane during the hypotonic swelling. The procedure was monitored by a dedicated flow cytometer for reticulocyte counting and required 120 min. Approximately 20% of the erythrocytes undergoing the reversible osmotic lysis were encapsulated with various amounts of RNA. The morphology of the RNA-loaded erythrocytes were similar to those of normal erythrocytes and reticulocytes, however, their mean cell volume (MCV) was slightly smaller than normal cells. RNA-loaded erythrocytes prepared by this method were stable for several months as a reference control for identification and enumeration of reticulocytes using flow cytometric as well as manual analysis methods and resulted in a high correlation coefficient between these counting techniques. © 1996 Wiley-Liss, Inc.
Databáze: OpenAIRE
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