An improved method for the determination of adenosinetriphosphate in environmental samples
| Autor: | R.S. Tobin, B.K. Afghan, J.F. Ryan |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
chemistry.chemical_classification
Environmental Engineering Chromatography Chloroform Ecological Modeling Improved method Pollution Metal chemistry.chemical_compound Hydrolysis Enzyme Adsorption Trisodium phosphate chemistry visual_art visual_art.visual_art_medium Humic acid Waste Management and Disposal Water Science and Technology Civil and Structural Engineering |
| Zdroj: | Water Research. 12:783-792 |
| ISSN: | 0043-1354 |
| DOI: | 10.1016/0043-1354(78)90028-3 |
| Popis: | An improved method has been developed for the analysis of the adenosinetriphosphate (ATP) content of environmental samples with the luciferin-luciferase assay. Factors affecting the recovery and stability of the ATP as well as the enzymatic reaction were optimized. The most versitile method utilizes procedure which can be used for natural waters, sediments and sludges. This involves homogenization at room temperature in 10−2 M trisodium phosphate, pH 11.7, containing 23% chloroform. This method is more effective than other methods tested and results in minimal interference with the enzymatic reaction. It also provides improved ATP recovery and long term stability of extracted ATP. Another method that can be used on natural waters and sludges is by boiling for 5 min in 10−2 M glycine buffer, 5 × 10−3 M Mg-EDTA, pH 10, which resulted in complete extraction and no hydrolytic loss of ATP. However, it is not satisfactory for sediments containing high humic acid substances as well as high metal loadings. In addition to the direct comparison of endogenous ATP extraction by various methods, actual recoveries of spiked radioactive ATP was measured. ATP adsorption, hydrolysis and recoveries in each method were determined by the use of [8-14C] ATP and [γ-32P] ATP. |
| Databáze: | OpenAIRE |
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