Development and Validation of an HPLC Method with Mass Spectrometric Detection for Quantitative Determination of Chlormadinone Acetate and Ethinyl Estradiol in Human Blood Plasma
| Autor: | A. V. Belostotskii, G. N. Gil’deeva, D. S. Sapozhnikova |
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| Rok vydání: | 2017 |
| Předmět: |
Pharmacology
Chromatography Human blood 010405 organic chemistry Chemistry Extraction (chemistry) 01 natural sciences High-performance liquid chromatography Quantitative determination 0104 chemical sciences 010404 medicinal & biomolecular chemistry Chlormadinone acetate chemistry.chemical_compound Pharmacokinetics Drug Discovery Sample preparation Hplc method hormones hormone substitutes and hormone antagonists |
| Zdroj: | Pharmaceutical Chemistry Journal. 51:142-152 |
| ISSN: | 1573-9031 0091-150X |
| DOI: | 10.1007/s11094-017-1573-8 |
| Popis: | An HPLC method with mass-spectrometric detection was developed for simultaneous quantitative determination of chlormadinone acetate and ethinyl estradiol in human blood plasma. The sample preparation method included liquid—liquid extraction using hexane—Et2O. The method was validated for selectivity, linearity, accuracy, precision, cross-transfer, and stability. The developed method enabled simultaneous determination of the chlormadinone acetate and ethinyl estradiol blood concentrations in volunteers and could be used for pharmacokinetics studies. |
| Databáze: | OpenAIRE |
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