Production of nitric oxide as related to cardiomyocyte injury upon regional myocardial ischemia and reperfusion in rats

Autor: L. I. Serebryakova, Alexander A. Timoshin, Oleg I. Pisarenko, D. Yu. Drobotova, Enno K. Ruuge, I. M. Studneva, Tskitishvili Ov
Rok vydání: 2008
Předmět:
Zdroj: Biophysics. 53:313-316
ISSN: 1555-6654
0006-3509
DOI: 10.1134/s0006350908040155
Popis: Changes in nitric oxide concentration in the rat myocardium in situ during temporary occlusion of the anterior descending coronary artery and subsequent reperfusion were monitored by microdialysis in the risk zone and a normal zone, using an NO trap (complex of ferrous ions with N-methyl-D-glucamine dithiocarbamate, Fe3+-MGD). The amplitude of the EPR signal of the reduced adduct NO-Fe2+-MGD in the samples from the risk zone increased during the 40-min occlusion and remained higher than the initial or the current control values during 60-min postischemic reperfusion, indicating substantial NO production. By the end of reperfusion, the infarct size was 47 ± 3% of the risk area. The contents of ATP, creatine phosphate, and total creatine in the risk zone decreased to respectively 44 ± 4, 51 ± 5, and 60 ± 3% of the initial values, whereas the level of lactate was six times the initial. The normal zone of the left ventricle showed no changes in NO or energy metabolite levels throughout the experiment. Thus, intense nitric oxide production in acute regional ischemia and reperfusion is associated with disturbance of energy metabolism, cell membrane damage, and death of cardiomyocytes.
Databáze: OpenAIRE
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