High-performance genetic analysis using microfabricated capillary array electrophoresis microplates

Autor:	Charles A. Emrich, Igor L. Medintz, James R. Scherer, Robert G. Blazej, Brian M. Paegel, Richard A. Mathies, Lorenzo Berti
Rok vydání:	2001
Předmět:	Analyte Electrophoresis Confocal Clinical Biochemistry Microsatellite Sample preparation Computational biology Biochemistry Genetic analysis Genotyping Molecular biology DNA sequencing Analytical Chemistry
Zdroj:	ELECTROPHORESIS. 22:3845-3856
ISSN:	1522-2683 0173-0835
DOI:	10.1002/1522-2683(200110)22:18<3845::aid-elps3845>3.0.co;2-0
Popis:	This review focuses on some recent advances in realizing microfabricated capillary array electrophoresis (microCAE). In particular, the development of a novel rotary scanning confocal fluorescence detector has facilitated the high-speed collection of sequencing and genotyping data from radially formatted microCAE devices. The concomitant development of a convenient energy-transfer cassette labeling chemistry allows sensitive multicolor labeling of any DNA genotyping or sequencing analyte. High-performance hereditary haemochromatosis and short tandem repeat genotyping assays are demonstrated on these devices along with rapid mitochondrial DNA sequence polymorphism analysis. Progress in supporting technology such as robotic fluid dispensing and batched data analysis is also presented. The ultimate goal is to develop a parallel analysis platform capable of integrated sample preparation and automated electrophoretic analysis with a throughput 10-100 times that of current technology.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c5438032993cf9aa15ce87e4c8c46d9e https://doi.org/10.1002/1522-2683(200110)22:18<3845::aid-elps3845>3.0.co Zobrazit plný text záznamu Plný text