FtsH facilitates proper biosynthesis of photosystem I in Arabidopsis thaliana
| Autor: | Marjaana Suorsa, Yagut Allahverdiyeva, Eva-Mari Aro, Sanna Rantala, Aiste Ivanauskaite, Dario Leister, Luca Tadini, Sari Järvi |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Photosynthetic reaction centre Photosystem II biology Physiology Plant Science biology.organism_classification Photosystem I 01 natural sciences 03 medical and health sciences Light intensity 030104 developmental biology Membrane protein Biochemistry Arabidopsis Thylakoid Genetics Biophysics Arabidopsis thaliana 010606 plant biology & botany |
| Zdroj: | Plant Physiology. :00200.2016 |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.16.00200 |
| Popis: | Thylakoid membrane-bound FtsH proteases have a well-characterized role in degradation of the photosystem II (PSII) reaction center protein D1 upon repair of photodamaged PSII. Here, we show that the Arabidopsis (Arabidopsis thaliana) var1 and var2 mutants, devoid of the FtsH5 and FtsH2 proteins, respectively, are capable of normal D1 protein turnover under moderate growth light intensity. Instead, they both demonstrate a significant scarcity of PSI complexes. It is further shown that the reduced level of PSI does not result from accelerated photodamage of the PSI centers in var1 or var2 under moderate growth light intensity. On the contrary, radiolabeling experiments revealed impaired synthesis of the PsaA/B reaction center proteins of PSI, which was accompanied by the accumulation of PSI-specific assembly factors. psaA/B transcript accumulation and translation initiation, however, occurred in var1 and var2 mutants as in wild-type Arabidopsis, suggesting problems in later stages of PsaA/B protein expression in the two var mutants. Presumably, the thylakoid membrane-bound FtsH5 and FtsH2 have dual functions in the maintenance of photosynthetic complexes. In addition to their function as a protease in the degradation of the photodamaged D1 protein, they also are required, either directly or indirectly, for early assembly of the PSI complexes. |
| Databáze: | OpenAIRE |
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