Dephosphorylation of cell-surface phosphoproteins of goat spermatozoa
| Autor: | Gopal C. Majumder, Madhabi Barua |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Metal ions in aqueous solution
Cell Endogeny General Medicine Biology Molecular biology Sperm General Biochemistry Genetics and Molecular Biology Bivalent (genetics) In vitro Spermidine Dephosphorylation chemistry.chemical_compound medicine.anatomical_structure chemistry medicine General Agricultural and Biological Sciences |
| Zdroj: | Journal of Biosciences. 15:217-221 |
| ISSN: | 0973-7138 0250-5991 |
| DOI: | 10.1007/bf02703888 |
| Popis: | Multiple ecto-phosphoproteins of the goat cauda-epididymal intact spermatozoa have been shown to undergo dephosphorylationin vitro by endogenous phosphoprotein phosphatase(s) located on the sperm outer surface. The major ecto-phosphoproteins that are dephosphorylated have molecular masses of 27, 40, 70, 116 and 205 kDa. The cell surface dephosphorylation reaction is not dependent on bivalent metal ions. Mg2+ (5 mM), Mn2+ (5 mM), orthovanadate (200ΜM) and cAMP (5 ΜM) have no effect on this surface reaction whereas it is inhibited nearly 50% by Co2+ or Zn2+ (1 mM). Spermidine (5 mM), or Ca2+ (1mM) inhibited to a small extent (approx. 25%) the cell surface dephosphorylation of proteins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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