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In this study, juvenile tropical freshwater fish (Oreochromis niloticus) were exposed to different lethal concentrations (2.5 ml/L, 5.0ml/L, 10.0ml/L, 15.0ml/L, 20.0ml/L and 25.0ml/L respectively) of aqueous methanol and (0ml/L) which is a tank without the toxicant to serve as the control. The experiment was conducted using a static nonrenewable bioassay method. The fish were obtained from the African Regional Aquaculture Centre (ARAC), Aluu, Rivers State, Nigeria. The fishes were acclimated to an aquarium for 14 days. In order to determine the definitive test concentration, a range-finding test was conducted. The mortality, LC50s value, and the 95 per cent confidence intervals for test organisms were derived using standard procedures at 24hr, 48hr, 72hr, and 96hr respectively. There was a statistically (P |
