Callus and root formation from mesophyll protoplasts of Brassica rapa

Autor:	Jack M. Widholm, J.B. Chowdhury, T.H. Ulrich
Rok vydání:	1980
Předmět:	Root formation fungi food and beverages Soil Science Cellulase Biology Protoplast Greening Callus Shoot Brassica rapa Botany biology.protein Pectinase Agronomy and Crop Science
Zdroj:	Plant Science Letters. 19:347-354
ISSN:	0304-4211
DOI:	10.1016/0304-4211(80)90057-7
Popis:	Protoplasts from mesophyll cells of turnip plants ( Brassica rapa L. cv. Just Right) were isolated by treating the peeled leaves with a mixture of cellulase and pectinase. Up to 36% of the protoplasts formed cell clumps in a liquid medium that was replenished weekly. Cell divisions began in 4 days and callus cells projected from aggregates in 14 days. Calli transferred from liquid to several solid media grew rapidly and in one medium root regeneration resulted. One liquid medium induced greening of calli, but no shoots were formed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c529038ccaf14515e36aae6b0bedecd7 https://doi.org/10.1016/0304-4211(80)90057-7 Zobrazit plný text záznamu