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Introduction Preeclampsia (PE) is a life-threatening complication of pregnancy associated with a high rate of maternal and perinatal morbidity and/or mortality worldwide. If untreated, it can progress to eclampsia, which can result in the death of mother, fetus or both. Objectives Etiology of PE is still uncertain, however recently the role of immune system takes on importance. Thus the role of central cytokine, tumor necrosis factor (TNF) involved in inflammation processes become widely investigated in obstetric disorders. Following this the present study is to investigate the effect of TNF-alpha gene G308A (rs1800629) polymorphism on disease risk, renal functions, microvascular permeability, endothelial cell dysfunction and organ involvement in women with preeclampsia. Methods Initially a 102 3 rd trimester pregnant women (preeclamptic-cases and healthy controls) with singleton pregnancy were invited for participation of which 76 were genotyped for TNF-alpha G308A polymorphism, evaluated for plasma levels of sVCAM-1, fibronectin, TNF-alpha and correlated to profile of preeclampsia. The odds ratio (OR) and 95% confidence intervals obtained from unconditional logistic regression were used to set association between TNF-apolymorphism and PE risk. For continuous variables we applied Student's t -test and for categorical variables the Pearson x 2 or Fisher's exact test. The two-way ANOVA with Bonferroni correction was used in multivariate analysis. Results The A allele was more frequent in cases than controls (22.4% vs. 13.2%) what increased disease risk (OR=2.73). Maternal serum levels of TNF-alpha, sVCAM-1 and fibronectin were significantly increased in cases (855.8±385.1pg/mL, 1243±671ng/mL, 0.308±0.231g/L) compared to controls (301.1±156.1pg/mL, 651±250ng/mL, 0.218±0.101g/L, p p p =0.031; respectively) and levels shoved increasing trend with mutant allele in genotype. Moderate and severe proteinuria was higher in rs1800629 allele A subjects compared to G/G carriers (53.8% vs. 14.3%, p p Conclusion This study revealed possible association between clinical and laboratory manifestations of preeclampsia and TNF-alpha gene G308A (rs1800629) polymorphism. |
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