| Autor: |
Hou Yanfei, Li Mingshun, Yu Ziniu, Liu Jinhui, Guo Hongsen |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Chinese Journal of Appplied Environmental Biology. 18:235 |
| ISSN: |
1006-687X |
| DOI: |
10.3724/sp.j.1145.2012.00235 |
| Popis: |
The nematicidal activities of 12 Bacillus thuringiensis strains against Ditylenchus destructor were tested to obtain nematicidal B.thuringiensis strains with high toxicity to D.destructor.The insecticidal crystal proteins(ICPs) were extracted from each B.thuringiensis strain and dissolved.The larva and adult of D.destructor were incubated with ICPs,and the mortalities of D.destructor were counted after 3 and 7 days.After primary and secondary screening,one Bt strain,YBT-008,which showed high toxicity to D.destructor,was obtained.The result of bioassay indicated that the estimated LC50of YBT-008 against D.destructor was 203.76 μg/mL.The primary characterization of strain YBT-008 was also analyzed.The growth curve showed that YBT-008 was in the stationary phase after 12 h incubation.The ovate parasopral crystals could be obviously observed by microscope.The electrophoresis result indicated that plasmids from YBT-008 displayed 5 bands.The SDS-PAGE analysis showed that YBT-008 produced a variety of unidentified proteins.The finding of YBT-008 strain would provide new strain and gene resources for the biological control against D.destructor. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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