Regulation of influenza virus-induced germinal center reactions (130.9)
| Autor: | Alexander Boyden, Kevin Legge, Thomas Waldschmidt |
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| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 182:130.9-130.9 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.182.supp.130.9 |
| Popis: | Previous work from our laboratory has demonstrated a steady state environment within primary splenic germinal center (GC) reactions as measured by the ratio of non-switched (IgM+) to switched (IgM-) GC B cells, following i.p. challenge with SRBC or protein Ags precipitated in alum. Since both of these experimental antigens induce a Th2-biased response, we sought to analyze the GC reaction after challenge with influenza virus, a natural pathogen that induces B cell activation under Th1 conditions. BALB/c mice were challenged i.p. or i.n. with the H1N1 PR8 virus and splenocytes subjected to multi-parameter flow cytometric analysis. After i.p. challenge, we again observed a steady ratio of IgM+ to switched GC B cells in the spleen. Surprisingly, an intense GC response was found in the spleen following respiratory (i.n.) challenge, with loss of a steady state environment at later time points. To evaluate the degree to which Tregs contribute to control of GC responses during influenza infection, mice were treated with anti-GITR or anti-CD25 mAb prior to and during viral challenge. Treg disruption resulted in a marked abrogation of GC homeostasis in the spleen, leading to exaggerated numbers of switched GC B cells. |
| Databáze: | OpenAIRE |
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