Effects of dietary purified rapeseed protein concentrate on hepatic gene expression in juvenile turbot (Psetta maxima)
| Autor: | Thorsten B. H. Reusch, B. T. Hermann, Reinhold Hanel |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Phytic acid Rapeseed biology 04 agricultural and veterinary sciences Aquatic Science biology.organism_classification Turbot 03 medical and health sciences Metabolic pathway chemistry.chemical_compound 030104 developmental biology chemistry Downregulation and upregulation Biochemistry Transferrin Gene expression 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Cytochrome c oxidase Food science |
| Zdroj: | Aquaculture Nutrition. 22:170-180 |
| ISSN: | 1353-5773 |
| DOI: | 10.1111/anu.12251 |
| Popis: | Despite considerable progress in the production of alternative diets, small concentrations of antinutrients remain common in aquaculture nutrition, resulting in a perpetual limitation with regard to the inclusion of plant ingredients in aquafeeds. These compounds are known to impair the general performance of fish when fed for a prolonged period of time, potentially affecting the animal's susceptibility to stress, too. Therefore, a 12-week feeding trial was conducted to examine the chronic effects of purified rapeseed protein concentrate (RPC), containing low concentrations of glucosinolates and phytic acid, on the relative expression of multiple target genes in the liver of juvenile turbot (Psetta maxima, L.). Our results revealed divergent patterns of gene expression, suggesting different coping strategies dependent on the grade of RPC substitution. Data implies increased metabolic rate of turbot fed a 33% RPC-substituted diet due to an upregulation of cytochrome c oxidase mRNA, accompanied by minor adjustments in metabolic pathways. While no signs of reduced welfare were found, data adumbrate a beneficial hormetic reaction. In the highest treatment level (66% RPC), diminished fish condition and reduced growth performance coincided with a downregulation of insulin-like growth factor I, further indicating a potential impaired resistance to stress. An additional downregulation of transferrin hints towards an increased liability to bacterial infections. |
| Databáze: | OpenAIRE |
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