| Autor: |
Lovisa Dousha, Belinda Thomas, Jane E. Bourke, Julia Chitty, Philip G. Bardin |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Airway cell biology and immunopathology. |
| DOI: |
10.1183/13993003.congress-2021.pa3691 |
| Popis: |
1. Precision cut lung slices (PCLS) offer a unique integrated experimental platform for investigating both inflammatory and immune responses to viral infection. 2. This study aimed to develop a protocol for robust infection of PCLS ex vivo with influenza A virus (IAV) with a view to its application for assessment of antiviral agents. 3. PCLS were prepared using agarose-inflated lungs from naive C57Bl6 mice and infected with IAV (HKx31 mouse strain). Matched slices from each mouse at 3 IAV concentrations (1x104-1x106 PFU) were assessed 24 and 48 hr post infection (n=4-8). Viral loads were assessed by plaque assay of homogenised PCLS. Conditioned media was used to measure both cell death via LDH assay and inflammatory responses to infection via TNFα ELISA. 4. A dose-dependent increase in viral load was observed at 24 hr, with a further 3-fold increase in plaque numbers at 48 hr at the lowest IAV concentration only (1x104PFU, p 5. Treatment of mouse PCLS with IAV ex vivo elicits dose- and time-dependent infection and release of inflammatory cytokines. Further studies assessing immune responses and sensitivity to viral treatment are required. PCLS may be a viable screening tool for pre-clinical assessment of mechanisms of infection and validation of new therapeutic targets. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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