3D perfusion bioreactor-activated porous granules on implant fixation and early bone formation in sheep
| Autor: | Susan Snoek Henriksen, Roberta Martinetti, Søren Overgaard, Ming Ding |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Materials science 0206 medical engineering Biomedical Engineering Synthetic graft 02 engineering and technology Fibrous tissue 020601 biomedical engineering Perfusion bioreactor Surgery Biomaterials Implant fixation 03 medical and health sciences surgical procedures operative 030104 developmental biology Bone graft materials medicine Bone formation Implant Biomedical engineering Fixation (histology) |
| Zdroj: | Journal of Biomedical Materials Research Part B: Applied Biomaterials. 105:2465-2476 |
| ISSN: | 1552-4973 |
| DOI: | 10.1002/jbm.b.33783 |
| Popis: | Early fixation of total joint arthroplasties is crucial for ensuring implant survival. An alternative bone graft material in revision surgery is needed to replace the current gold standard, allograft, seeing that the latter is associated with several disadvantages. The incubation of such a construct in a perfusion bioreactor has been shown to produce viable bone graft materials. This study aimed at producing larger amounts of viable bone graft material (hydroxyapatite 70% and β-tricalcium-phosphate 30%) in a novel perfusion bioreactor. The abilities of the bioreactor-activated graft material to induce early implant fixation were tested in a bilateral implant defect model in sheep, with allograft as the control group. Defects were bilaterally created in the distal femurs of the animals, and titanium implants were inserted. The concentric gaps around the implants were randomly filled with either allograft, granules, granules with bone marrow aspirate or bioreactor-activated graft material. Following an observation time of 6 weeks, early implant fixation and bone formation were assessed by micro-CT scanning, mechanical testing, and histomorphometry. Bone formations were seen in all groups, while no significant differences between groups were found regarding early implant fixation. The microarchitecture of the bone formed by the synthetic graft materials resembled that of allograft. Histomorphometry revealed that allograft induced significantly more bone and less fibrous tissue (p |
| Databáze: | OpenAIRE |
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