Inactivation of Bacillus Subtilis Spores by a Combination of Hydrostatic High-Pressure and Pulsed Electric Field Treatments

Autor:	Atsushi Kobayashi, Masayuki Sato, Jun Hoshino, Akifumi Yamada, Takayuki Ohshima, Tomoyuki Fujii, Akihiko Sasagawa, Akira Yamazaki
Rok vydání:	2006
Předmět:	Log reduction biology Chemistry fungi General Chemistry Bacillus subtilis Sterilization (microbiology) Condensed Matter Physics biology.organism_classification Spore Germination Electric field High pressure General Materials Science Food science
Zdroj:	The Review of High Pressure Science and Technology. 16:45-53
ISSN:	1348-1940 0917-639X
DOI:	10.4131/jshpreview.16.45
Popis:	The effect of the combination of hydrostatic high-pressure (HHP) and pulsed electric field (PEF) treatments on the inactivation of Bacillus subtilis spores was investigated, using suspensions prepared by suspending the spores in various solutions at about 108 spores/ml. When the PEF and HHP treatments were successively conducted in that order, the results were compared with the case where either was conducted alone. The combined processing achieved a 7.1 log reduction in the viable spore counts at maximum. Low-temperature storage after the combined processing further led to reduced germination, and finally completes inactivation in 3 days. It was confirmed by phase-contrast microscopy that the non-treated spores gradually turned into phase-dark spores and finally germinated and changed into vegetative cells, while the spores subjected to PEF/HHP treatment did not transfer to the phase-dark stage, meaning no germination.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::c4cc9e984b162e65eac88061f0e47f18 https://doi.org/10.4131/jshpreview.16.45 Zobrazit plný text záznamu