Liquid chromatography tandem mass spectrometry for the quantification of steroid hormone profiles in blubber from stranded humpback whales (Megaptera novaeangliae)
| Autor: | Susan Bengtson Nash, Greta Dalle Luche, Fletcher M. J. Mingramm, John R. Kucklick, Ashley S.P. Boggs |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
biology Physiology 010604 marine biology & hydrobiology Ecological Modeling medicine.medical_treatment Physiological condition Management Monitoring Policy and Law biology.organism_classification 010603 evolutionary biology 01 natural sciences Baleen whale Steroid hormone Marine mammal Blubber medicine Androstenedione Testosterone Nature and Landscape Conservation Hormone |
| Zdroj: | Conservation Physiology. 7 |
| ISSN: | 2051-1434 |
| Popis: | Marine mammal blubber is known to have quantifiable concentrations of steroid hormones and is increasingly chosen as a matrix for the detection of these reproductive and stress biomarkers. Steroid hormones act through complex cascades, often in concert, yet studies conducted on cetaceans have rarely measured more than two steroids simultaneously. Due to the role of steroid hormones in multiple physiological processes, and variability in concentration among individuals, data on single compounds are often difficult to interpret. Here a liquid chromatography tandem mass spectrometry method for the simultaneous analyses of multiple steroid hormones in cetacean blubber was validated and applied to samples from 10 stranded humpback whales (Megaptera novaeangliae). Progesterone, 17α-hydroxyprogesterone, testosterone, androstenedione, oestrone, oestradiol, cortisone, cortisol, corticosterone and 11-deoxycorticosterone were reliably (relative standard deviation on six replicates |
| Databáze: | OpenAIRE |
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