Muscle phenotype remains unaltered after limb autotomy and unloading
| Autor: | Stacia B. Moffett, Robin L. Cooper, Bruce Griffis |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Procambarus clarkii
medicine.medical_specialty General Medicine Anatomy Biology biology.organism_classification medicine.disease Phenotype Tonic (physiology) Major duodenal papilla Atrophy Endocrinology Internal medicine Myosin medicine Excitatory postsynaptic potential Animal Science and Zoology Autotomy |
| Zdroj: | Journal of Experimental Zoology. 289:10-22 |
| ISSN: | 1097-010X 0022-104X |
| DOI: | 10.1002/1097-010x(20010101/31)289:1<10::aid-jez2>3.0.co;2-r |
| Popis: | Loss of chelipeds in crustaceans results in severe atrophy of the major muscle responsible for lifting the limb, the anterior levator. We decided to test if this loss of mechanical load altered muscle phenotype as measured by SDS-PAGE analysis of levator total protein and actomyosin fractions. Levator muscles of adult crayfish, Procambarus clarkii, with either func- tional regenerate limbs or lack of limb buds (papilla stage) were compared with those from normal contralateral limbs and those from pristine animals. We find that there is no difference in protein profiles among the three conditions. However, the total protein profile for the dually excited leva- tor muscle is unique compared to those of fast or slow muscles of the abdomen (L and SEL, respec- tively), which receive only phasic or tonic excitatory innervation. The levator myosin heavy chain profile is similar to that of slow phenotype muscles such as the SEL and opener. We conclude that load does not influence levator phenotype. This is likely due either to the intact innervation and continued activation of the levator during atrophy or to the maintenance of passive tension on the muscle. J. Exp. Zool. 289:10-22, 2001. © 2001 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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